Pyridovericin and pyridomacrolidin: novel metabolites from entomopathogenic fungi, Beauveria bassiana.

program, we systematically investigated the diversity of metabolites in Deuteromycota, Beauveria bassiana, Metarhizium anisopliae, Nomuraea rileyi, Paecilomyces fumosoroseus and Verticillium lecanii, which have been isolated from the corpses of insects. Comparative study of the HPLC elution pattern among fungal metabolites found that a strain, Beauveria bassiana EPF-5, produced two novel metabolites, designated pyridovericin (1) and pyridomacrolidin (2). In this communication, we preliminarily report the production, isolation, structure and biological activities of 1 and 2. The detection of fungal metabolites by HPLC analysis was performed under the following conditions: column: ODS column (Senshu Pak PEGASIL); solvent: a linear gradient 50% MeOH to 70% MeOH for 50 minutes; UV detection at 220nm, 254nm and 310nm; flow rate of 3.0ml/minute. Beauveria bassiana EPF-5, a strain isolated from an adult Mulberry small weevil (Baris